Leica Sr Gsd 3d

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Leica sr gsd 3d. Leica microsystems are pioneers in super resolution microscopy. Leica sr gsd 3d gsd ground state depletion products leica microsystems. The leica sr gsd 3d is equipped with a 160x high performance objective specifically developed for super resolu tion microscopy. Replaced by leica sr gsd 3d.

Its design is optimized for high power laser emissions. Quantifying the resolution of a leica sr gsd 3d localization microscopy system with 2d and 3d nanorulers measuring the nano world updated version dna origami based nanorulers produced by gattaquant are the perfect positive control samples that tell you in a few minutes the status of your microscope and help you to test settings and configurations of your super resolution microscope. Leica sr gsd 3d. Redefine the limits of microscopy widefield super resolution with ground state depletion.

We were the first to test the leica astigmatic lens solution for 3d imaging movie 2. The extremely low autofluorescence ensures a high signal to noise ratio which is ideal for single molecule detection applications. Technical data september 2017. Leica sr gsd 3d.

The leica sr gsd 3d system is a new approach in localization microscopy for showing cellular compartments with high resolution in the third dimension. In this protocol guide we will focus on preparation of samples used for super resolution microscopy. The leica sr gsd 3d system has sectioning properties itself so it is possible to combine different focal planes routinely with the help of the oxygen tight chamber movie 1. Sample preparation for 2d and 3d imaging are identical.

Maximum resolution in z down to 50 nm. Maximum resolution in xy down to 20 nm. The 2007 introduction of the leica tcs sted heralded a new era of products that break the barriers of diffractionlimited imaging. Gsdim is a scientifically proven method using a wide range of standard fluorochromes.

Leica sr gsd 3d technical brochure en 20170922 pdf 725 kb leica sr gsd technical brochure cn 20111012 pdf 1 mb leica sr gsd technical brochure en 20130506 pdf 483 kb. To understand transport processes in polarized epithelial cells the exact localization of different cellular organelles involved in protein trafficking is necessary. Leica sr gsd 3d puts the power of high resolution into a user friendly system along with a full range of widefield microscopy applications. As a multi functional system it gives researchers the freedom to tailor the system exactly to their applications in live cell or advanced fluorescence imaging.

From eye to insight.